Although image registration will work with any type of image, it has been optimized for automatically aligning 2D protein gels. Aligning and measuring the hundreds of spots on a typical 2D gel can be tedious and time-consuming. Below is a procedure for performing densitometry on two 2D gels rapidly.
There are two ways of analyzing 2D gels in imal. Use the first way if you want to measure all the spots. Use the second way if you are only interested in the spots that are different between a control and an experimental gel (as in DIGE).